Promos
| Artist | Album | Song |
| Dave Matthews Band | Stand Up | Out of My Hands |
| Crossfade | Crossfade | No Giving Up |
Unaired Pilot
| Artist | Album | Song |
| Green Day | American Idiot | Boulevard of Broken Dreams |
| Sam & Jessica get ready for the party | ||
| 50 Cent | The Massacre | Disco Inferno |
| Jessica & Sam celebrate at the party | ||
| Jay Z | The Black Album | 99 Problems |
| Troy meets the woman in white in his car | ||
| ZZ Top | Tres Hombres | La Grange |
| Dean and Sam at the gas station | ||
| Metallica | Metallica | Enter Sandman |
| Dean and Sam on the road after leaving the gas station | ||
| AC/DC | Highway to Hell | Highway to Hell |
| Dean & Sam head out of Jericho and back to Stanford | ||
| AC/DC | Back in Black | Back in Black |
| Closing credits | ||
Pilot
| Artist | Album | Song |
| The Living Daylights | Gasoline | |
| Sam & Jessica get ready for the party | ||
| Classic | What Cha Gonna Do | |
| Jessica & Sam celebrate at the party | ||
| Eagles of Death Metal | Peace Love Death Metal | Speaking in Tongues |
| Troy meets the woman in white in his car | ||
| Allman Brothers Band | Brothers & Sisters | Ramblin' Man |
| Dean and Sam at the gas station | ||
| AC/DC | Back in Black | Back in Black |
| Dean and Sam on the road after leaving the gas station | ||
| AC/DC | Highway to Hell | Highway to Hell |
| Dean & Sam head out of Jericho and back to Stanford | ||
| Kid Gloves Music | My Cheatin' Ways | |
Wendigo
| Artist | Album | Song |
| Foreigner | Double Vision | Hot Blooded |
| Sam awakes from his nightmare in the car | ||
| Lynyrd Skynyrd | Double Trouble | Down South Jukin' |
| Plays in the bar as Sam & Dean discuss the disappearances | ||
| Rush | Fly By Night | Fly By Night |
| Sam tells Dean that he's driving and they take off | ||
Dead in the Water
| Artist | Album | Song |
| Black Toast | What a Way to Go | |
| Dean & Sam discuss the Lake Manitoc disappearances at the diner | ||
| Ratt | Out of the Cellar | Round and Round |
| The brothers drive to Lake Manitoc | ||
| Billy Squier | Don't Say No | Too Daze Gone |
| While driving, Dean & Sam discuss the drawing Lucas gave Dean | ||
| Bad Company | Bad Company | Movin' On |
| The boys say goodbye to Lucas & Andrea and head out | ||
Phantom Traveler
| Artist | Album | Song |
| Black Sabbath | Paranoid | Paranoid |
| The brothers pretend to work for Homeland Security in their snazzy new suits | ||
| Rush | Rush | Working Man |
| Dean & Sam drive to the airport to try to stop Amanda getting on the plane | ||
Bloody Mary
| Artist | Album | Song |
| Fall Out Boy | From Under the Cork Tree | Sugar, We're Goin Down |
| Jill gets a visit from Bloody Mary | ||
| Def Leppard | Pyromania | Rock of Ages |
| Dean & Sam drive back from Fort Wayne & discuss the mirror | ||
| The Rolling Stones | A Bigger Bang | Laugh, I Nearly Died |
| Sam tells Charlie that her boyfriend's death wasn't her fault | ||
Skin
| Artist | Album | Song |
| Iron Butterfly | In-A-Gadda-Da-Vida | In-A-Gadda-Da-Vida |
| Opening of the episode as Shapeshifter!Dean is caught at Rebecca's | ||
| Lynyrd Skynyrd | Pronounced Leh-Nerd Skin-Nerd | Poison Whiskey |
| Dean & Sam at the gas station | ||
| Filter | Short Bus | Hey Man, Nice Shot |
| Shapeshifter!Dean runs from the police and sheds his Dean skin | ||
| Free | Fire and Water | All Right Now |
| Dean & Sam head out of town | ||
Hook Man
| Artist | Album | Song |
| Split Habit | Merry Go Round | |
| Lori asks her roommate for fashion advice | ||
| Quiet Riot | Quiet Riot - Greatest Hits | Bang Your Head (Metal Health) |
| Dean & Sam drive to Ankeny, Iowa | ||
| Low Five | Noise | |
| Dean & Sam talk to one of the frat boys about the murder | ||
| APM | At Rest | |
| Music in the church | ||
| APM | Royal Bethlehem | |
| Music in the church | ||
| Paul Richards | U Do 2 Me | |
| Music at the college party | ||
| Boston | Boston | Peace of Mind |
| Sam says goodbye to Lori & the brothers drive off | ||
Bugs
| Artist | Album | Song |
| Def Leppard | Pyromania | Rock of Ages |
| Dean wins money at pool & discusses their next case with Sam | ||
| Scorpions | Love at First Sting | Rock You Like a Hurricane |
| End of the episode as Dean & Sam hit the road | ||
Asylum
| Artist | Album | Song |
| Bachman-Turner Overdrive | Takin' Care of Business | Hey You |
| Dean & Sam talk to the cop in the bar | ||
Scarecrow
| Artist | Album | Song |
| Creedence Clearwater Revival | Chronicle, Vol. 1 | Lodi |
| In the bus station when Sam runs into Meg again | ||
| Bad Company | Bad Company | Bad Company |
| Meg kills the driver and makes her "call" | ||
Faith
| Artist | Album | Song |
| Blue Öyster Cult | Agents of Fortune | (Don't Fear) The Reaper |
| The reaper chases the jogger through the woods | ||
Route 666
| Artist | Album | Song |
| James Gang (Joe Walsh) | Little Did He Know | Walk Away |
| Dean gets a message from an old friend & tells Sam they're not going to Pennsylvania | ||
| Bad Company | Desolation Angels | She Brings Me Love |
| Dean and Cassie "work things out" | ||
| Blind Faith | Blind Faith | Can't Find My Way Home |
| Dean says goodbye to Cassie and the brothers head out | ||
Nightmare
| Artist | Album | Song |
| The Bob Seger System | Ramblin' Gamblin' Man | 2 + 2 = ? |
| Plays in the car of the first victim as he drives home | ||
The Benders
| Artist | Album | Song |
| Joe Walsh | Little Did He Know | Rocky Mountain Way |
Shadow
| Artist | Album | Song |
| Little Charlie and the Nightcats | Shadow of the Blues | You Got Your Hooks Into Me |
| Plays in the headphones of the victim as she walks home | ||
| Vue | Find Your Home | Pictures of Me |
| Plays in the bar when Sam & Dean run into Meg | ||
Hell House
| Artist | Album | Song |
| Blue Öyster Cult | Fire of Unknown Origin | Fire of Unknown Origin |
| Dean plays his first prank on a sleeping Sam in the car | ||
| Blue Öyster Cult | Fire of Unknown Origin | Burnin' For You |
| Dean & Sam interview the teens at the Rodeo Drive Dean & Sam agree to a truce and drive off | ||
Something Wicked
| Artist | Album | Song |
| UFO | Phenomenon | Rock Bottom |
| Dean & Sam discuss the coordinates sent to them by their father | ||
| Ozzy Osbourne | No More Tears | Road to Nowhere |
| Dean & Sam talk about Michael's loss of innocence and drive off | ||
Provenance
| Artist | Album | Song |
| Grand Funk Railroad | All the Girls in the World Beware | Bad Time |
| Dean plays this song to urge Sam to make his move on Sarah | ||
Dead Man's Blood
| Artist | Album | Song |
| Stevie Ray Vaughan & Double Trouble | In Step | The House is Rockin' |
| Elkins spots the vampires in the bar | ||
| Tito & Tarantula | Tarantism | Strange Face |
| The vampires taunt the couple they captured & Luther arrives | ||
Salvation
| Artist | Album | Song |
| Kansas | Leftoverture | Carry On Wayward Son |
| Previously on Supernatural... | ||
Devil's Trap
| Artist | Album | Song |
| Triumph | Allied Forces | Fight the Good Fight |
| Previously on Supernatural... | ||
| Barnstorm (Joe Walsh) | Little Did He Know | Turn to Stone |
| Dean & Sam flee Salvation and discuss what to do about their father | ||
| Creedence Clearwater Revival | Chronicle, Vol. 1 | Bad Moon Rising |
| End of the episode in the car | ||