In My Time of Dying
| Artist | Album | Song |
| Ted Nugent | Ted Nugent | Stranglehold |
| Then and Now... | ||
| Creedence Clearwater Revival | Chronicle, Vol. 1 | Bad Moon Rising |
| Playing in the car as the trucker surveys the damage | ||
Everybody Loves a Clown
| Artist | Album | Song |
| The Chambers Brothers | The Time Has Come | Time Has Come Today |
| Then and Now... | ||
| Three Dog Night | The Complete Hit Singles | Shambala |
| Playing on the radio as Dean works on the Metallicar | ||
| Captain & Tennille | Ultimate Collection: The Complete Hits | Do That to Me One More Time |
| Playing in the "soccer mom" van as Dean & Sam drive to the Roadhouse | ||
Bloodlust
| Artist | Album | Song |
| Journey | Infinity | Wheel in the Sky |
| Then and Now... | ||
| AC/DC | Back in Black | Back in Black |
| Return of the Metallicar! | ||
| Long John Hunter | Swinging From the Rafters | Time and Time Again |
| Playing in the bar where Sam & Dean first run into Eli | ||
| Lil Ed and the Blues Imperials | Rattleshake | Golden Rule |
| Sam and Dean drink with Gordon at a bar | ||
| Lee Rocker | Racin' the Devil | Funny Car Graveyard |
| Sam calls Ellen to inquire about Gordon | ||
Simon Said
| Artist | Album | Song |
| Daniel May | Woman's Wear | |
| Doc shoots the store owner and himself in Sam's vision | ||
| Lil Ed and the Blues Imperials | Rattleshake | Tired of Crying |
| Plays in the Roadhouse as the brothers look for Ash | ||
| REO Speedwagon | Wheels Are Turnin' | Can't Fight This Feeling |
| Jo's jukebox song of choice | ||
| Spinal Tap | This is Spinal Tap | Stonehenge |
| Dean and Sam stake out Andy's place while Dean admires the van | ||
| Soundgarden | Superunknown | Fell On Black Days |
| Playing in the bar as Dean & Sam tell Ellen about the other children | ||
No Exit
| Artist | Album | Song |
| Cheap Trick | Heaven Tonight | Surrender |
| Dean and Sam drive to and arrive in Philadelphia | ||
| Foreigner | Foreigner | Cold as Ice |
| Dean turns on the car radio on the way home; Ellen turns if off immediately | ||
Crossroad Blues
| Artist | Album | Song |
| Robert Johnson | King of the Delta Blues | Crossroad Blues |
| Rosedale, Missippippi, 1930: Robert makes his deal with the demon | ||
| Son House | The Original Delta Blues | Downhearted Blues |
| Dean and Sam talk to George Darrow about his deal | ||
| Big Bill Broonzy | The Big Bill Broonzy Story | Key to the Highway |
| Driving away in the Impala, Dean and Sam discuss their dad's death | ||
| Nazareth | Hair of the Dog | Hair of the Dog |
| Soon (preview) | ||
Hunted
| Artist | Album | Song |
| Jefferson Airplane | Surrealistic Pillow | White Rabbit |
| Played in the teaser when Scott talks to the doctor about his abilities, and as he walks to his car & is murdered | ||
| Carey Bell | Deep Down | Lonesome Stranger |
| Plays in the Roadhouse as Sam talks to Ellen | ||
| Muse | Black Holes & Revelations | Supermassive Black Hole |
| Ash gives the names of the other psychics to Sam | ||
Night Shifter
| Artist | Album | Song |
| Styx | Pieces of Eight | Renegade |
| The brothers escape from the bank and drive off | ||
Houses of the Holy
| Artist | Album | Song |
| Jamie Dunlap | Down on Love | |
| Dean rocks out while enjoying the "Magic Fingers" bed | ||
| Bob Dylan | Pat Garrett & Billy the Kid | Knockin' on Heaven's Door |
| Sam and Dean discuss Father Gregory and other issues of faith | ||
Born Under a Bad Sign
| Artist | Album | Song |
| Tarbox Ramblers | A Fix Back East | Ashes to Ashes |
| Sam visits the bar where Jo now works | ||
| The Doors | The Best of the Doors | The Crystal Ship |
| Sam ties Jo up and tells her about her father's death | ||
| REO Speedwagon | Nine Lives | Back on the Road Again |
| Plays on the car radio as Dean and Sam discuss Sam's possession | ||
Tall Tales
| Artist | Album | Song |
| James Gang (Joe Walsh) | Little Did He Know | Walk Away |
| Sam attempts to research using books as Dean eats on Sam's bed | ||
| Junk Food | Next to You | |
| Plays in the bar as Sam talks to the students and Dean hangs out with Starla | ||
| Chris de Burgh | Lady in Red | |
| Curtis slows dances with his alien captor | ||
| Barry White | Can't Get Enough | Can't Get Enough of Your Love, Babe |
| Dean enters the auditorium in search of the Trickster, but finds a love nest instead | ||
Roadkill
| Artist | Album | Song |
| The Animals | Retrospective | House of the Rising Sun |
| Plays multiple times over the radio as Greeley hunts Molly | ||
Heart
| Artist | Album | Song |
| Kip Winger | Smoking Gun | |
| Playing in the strip club as Dean tails Kurt | ||
| The Stooges | Funhouse | Down on the Street |
| Dean comes upon Madison killing Kurt and is knocked out by her | ||
| Screaming Trees | Dust | Look at You |
| Sam and Madison have make love | ||
| Queensryche | Empire | Silent Lucidity |
| Dean offers to kill Madison in place of Sam, but Sam insists upon doing it for her | ||
Hollywood Babylon
| Artist | Album | Song |
| Frank Sinatra | Classic Sinatra: His Great Performances | I've Got the World on a String |
| Dean and Sam go visit "Frank" aka Gerard St. James | ||
| Herb Alpert's Tijuana Brass | Whipped Cream and Other Delights | Green Peppers |
| Sam runs into Dean exiting Tara's trailer and the brothers head off into the "sunset" | ||
Folsom Prison Blues
| Artist | Album | Song |
| Booker T. & The MG's | Green Onions | Green Onions |
| Sam and Dean arrive at Green River Detention Center | ||
| Alice in Chains | Dirt | Rooster |
| The boys salt & burn Nurse Glockner while the FBI looks in the wrong cemetery | ||
What Is and What Should Never Be
| Artist | Album | Song |
| Lynyrd Skynyrd | Lynyrd Skynyrd: All Time Greatest Hits | Saturday Night Special |
| Sam and Dean discuss the Djinn over the phone Dean and Sam arrive at the warehouse in Illinois | ||
| Joey Ramone | Don't Worry About Me | What a Wonderful World |
| Dean mows the lawn for Mary | ||
All Hell Breaks Loose, Part 1
| Artist | Album | Song |
| Boston | Boston | Foreplay / Long Time |
| The Road So Far; Sam heads into the diner | ||
| Martyn Laight | ||
| Plays in the diner as Dean searches for Sam | ||
All Hell Breaks Loose, Part 2
| Artist | Album | Song |
| Kansas | Leftoverture | Carry On Wayward Son |
| The Road So Far | ||
| Boston | Don't Look Back | Don't Look Back |
| End of the episode: "We got work to do." | ||
